Journal
DIGESTIVE DISEASES AND SCIENCES
Volume 52, Issue 3, Pages 628-642Publisher
SPRINGER
DOI: 10.1007/s10620-006-9608-0
Keywords
colitis; nitric oxide synthase; deoxycholate; inflammatory bowel disease; colon cancer
Categories
Funding
- NCI NIH HHS [1R21CA111513-01A1, 1P50CA95060-01, P01 CA072008, CA72008, CA23074] Funding Source: Medline
Ask authors/readers for more resources
Nos2 knockout mice were compared to wild-type mice for susceptibility to colitis in response to a diet supplemented with deoxycholate, a bile acid increased in the colon of individuals on a high-fat diet. Wild-type mice fed a fat-related diet, supplemented with 0.2% DOC, develop colonic inflammation associated with increases in nitrosative stress, proliferation, oxidative DNA/RNA damage, and angiogenesis, as well as altered expression of numerous genes. However, Nos2 knockout mice fed a diet supplemented with deoxycholate were resistant to these alterations. In particular, 35 genes were identified whose expression was significantly altered at the mRNA level in deoxycholate-fed Nos2(+/+) mice but not in deoxycholate-fed Nos2(-/-) mice. Some of these alterations in NOS2-dependent gene expression correspond to those reported in human inflammatory bowel disease. Overall, our results indicate that NOS2 expression is necessary for the development of deoxycholate-induced colitis in mice, a unique dietary-related model of colitis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available