An antifungal peptide from red lentil seeds

An antifungal peptide, with a molecular mass of 11 kDa, was isolated from dry seeds of the red lentil (Lens culinaris) using a procedure that involved four chromatographic steps. The antifungal peptide was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and S-Sepharose. The final chromatographic step involved gel filtration by fast protein liquid chromatography on Superdex 75. The antifungal peptide inhibited mycelial growth in Mycosphaerella arachidicola with an IC50 of 36 mu M. It also exhibited antifungal activity against Fusarium oxysporum, but there was no inhibitory activity toward tumor cell lines and human immunodeficiency virus type 1 reverse transcriptase (RT). (c) 2006 Published by Elsevier Inc.