Journal
MOLECULAR MICROBIOLOGY
Volume 63, Issue 5, Pages 1399-1413Publisher
WILEY
DOI: 10.1111/j.1365-2958.2007.05588.x
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Funding
- Biotechnology and Biological Sciences Research Council [BB/C510191/1] Funding Source: Medline
- Medical Research Council [G0400284(70738), G0400284] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BB/C510191/1] Funding Source: researchfish
- Medical Research Council [G0400284] Funding Source: researchfish
- MRC [G0400284] Funding Source: UKRI
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Chitin is an essential component of the fungal cell wall and its synthesis is under tight spatial and temporal regulation. The fungal human pathogen Candida albicans has a four member chitin synthase gene family comprising of CHS1 (class II), CHS2 (class I), CHS3 (class IV) and CHS8 (class I). LacZ reporters were fused to each CHS promoter to examine the transcriptional regulation of chitin synthesis. Each CHS promoter had a unique regulatory profile and responded to the addition of cell wall damaging agents, to mutations in specific CHS genes and exogenous Ca2+. The regulation of both CHS gene expression and chitin synthesis was co-ordinated by the PKC, HOG MAP kinase and Ca2+/calcineurin signalling pathways. Activation of these pathways also resulted in increased chitin synthase activity in vitro and elevated cell wall chitin content. Combinations of treatments that activated multiple pathways resulted in synergistic increases in CHS expression and in cell wall chitin content. Therefore, at least three pathways co-ordinately regulate chitin synthesis and activation of chitin synthesis operates at both transcriptional and post-transcriptional levels.
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