Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 63, Issue -, Pages 204-208Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309107004903
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IREM-1 is an inhibitory receptor involved in the functional regulation of myeloid cells. The expression, in vitro folding, purification, crystallization and X-ray data collection of the Ig-V like domain of IREM-1 are reported. X-ray data were collected from a microcrystal ( 300 x 10 x 10 mm) at 100 K and a diffraction pattern was obtained to 2.6 angstrom resolution on microfocus beamline ID23-2 at the ESRF. The crystal belongs to space group P3(1)21, with unit-cell parameters a = b = 54.23, c = 72.02 angstrom, alpha = gamma = 90, beta = 120 degrees. Assuming the presence of one molecule per asymmetric unit, VM ( the Matthews coefficient) was calculated to be 1.96 angstrom(3) Da(-1) and the solvent content was estimated to be 37.27%. Determination of the IREM-1 structure will provide insights into its structural requirements for ligand discrimination and binding.
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