Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 140, Issue 1-2, Pages 75-79Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2006.11.001
Keywords
dengue; antigen detection; diagnostic; ELISA
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A commercial dengue NSI antigen-capture ELISA was evaluated to demonstrate its potential application for early laboratory diagnosis of acute dengue virus infection. Dengue Virus NSI antioen was detected in 199 of 213 acute serum samples from patients with laboratory confirmation of acute dengue virus infection but none of the 354 healthy blood donors' serum specimens. The dengue NSI antigen-capture ELISA gave an overall sensitivity of 93.4% (19/213) and a specificity of 100% (354/354). The sensitivity was significantly higher in acute primary dengue (97.3%) than in acute secondary dengue (70.0%). The positive predictive value of the dengue NS I antigen-capture ELISA was 100% and negative predictive value was 97.3%. Comparatively, virus isolation gave an overall positive isolation rate of 68.1% with a positive isolation rate of 73.9 and 31.0% for acute primary dengue and acute secondary dengue, respectively. Molecular detection of dengue RNA by RT-PCR gave an overall positive detection rate of 66.7% with a detection rate of 65.2 and 75.9% for acute primary dengue and acute secondary dengue, respectively. The results indicate that the commercial dengue NSI antigen-capture ELISA may be superior to virus isolation and RT-PCR for the laboratory diagnosis of acute dengue infection based on a single serum sample. (c) 2006 Elsevier B.V. All rights reserved.
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