Journal
ACTA BIOLOGICA HUNGARICA
Volume 58, Issue 1, Pages 75-86Publisher
AKADEMIAI KIADO ZRT
DOI: 10.1556/ABiol.57.2007.1.7
Keywords
molecular weight; chitosan; antibacterial activity; antibacterial mechanism; housefly larvae
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Chitosan was obtained from cuticles of the housefly (Musca domestica) larvae. Antibacterial activities of different Mw chitosans were examined against six bacteria. Antibacterial mechanisms of chitosan were investigated by measuring permeability of bacterial cell membranes and observing integrity of bacterial cells. Results show that the antibacterial activity of chitosan decreased with increase in Mw. Chitosan showed higher antibacterial activity at low pH. Ca2+ and Mg2+ could markedly reduce the antibacterial activity of chitosan. The minimum inhibitory concentrations of chitosans ranged from 0.03% similar to 0.25% and varied with the type or bacteria and Mw of chitosan. Chitosan could cause leakage of cell contents of the bacteria and disrupt the cell wall.
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