Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 55, Issue 3, Pages 263-274Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.6A7097.2006
Keywords
Helicobacter felis; insulin-gastrin transgenic mouse; high endothelial venule-like vessel; peripheral lymph node addressin; inflammation-carcinoma sequence; carcinogenesis; surfactant protein D; CD74
Categories
Funding
- NCI NIH HHS [CA-33000, CA-71932] Funding Source: Medline
- NIGMS NIH HHS [GM-62116] Funding Source: Medline
Ask authors/readers for more resources
Helicobacter pylori infects over half the population worldwide and is a leading cause of chronic gastritis and gastric cancer. However, the mechanism by which this organism induces inflammation and carcinogenesis is not fully understood. In the present study we used insulin-gastrin (INS-GAS) transgenic mice that fully develop gastric adenocarcinoma after infection of H. pylori-related Helicobacter felis. Histological examination revealed that more than half of those mice developed invasive adenocarcinoma after 8 months of infection. These carcinomas were stained by NCC-ST-439 and HECA-452 that recognize 6-sulfated and non-sulfated sialyl Lewis X. Lymphocytic infiltration predominantly to submucosa was observed in most H. felis-infected mice, and this was associated with the formation of peripheral lymph node addressin (PNAd) on high endothelial venule (HEV)-like vessels detected by MECA-79. Time-course analysis of gene expression by using gene microarray revealed upregulation of several inflammation-associated genes including chemokines, adhesion molecules, surfactant protein D (SP-D), and CD74 in the infected stomach. Immunohistochemical analysis demonstrated that SP-D is expressed in hyperplasia and adenocarcinoma whereas CD74 is expressed in adenocarcinoma in situ and invasive carcinoma. These results as a whole indicate that H. felis induces HEV-like vessels and inflammation-associated chemokines and chemokine receptors, followed by adenocarcinoma formation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available