Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 3, Pages 397-404Publisher
SPRINGER
DOI: 10.1007/s10529-006-9252-y
Keywords
lactate; ethanol; alkali metals; betaine; xylose; glucose; fermentation
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Individual nutrient salts were experimentally varied to determine the minimum requirements for efficient L(+)-lactate production by recombinant strains of Escherichia coli B. Based on these results, AM1 medium was formulated with low levels of alkali metals (4.5 mM and total salts (4.2 g l(-1)). This medium was equally effective for ethanol production from xylose and lactate production from glucose with average productivities of 18-19 mmol l(-1) h(-1) for both (initial 48 h of fermentation).
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