Journal
DEVELOPMENTAL CELL
Volume 12, Issue 3, Pages 457-465Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2007.02.010
Keywords
-
Categories
Ask authors/readers for more resources
All pancreatic endocrine cells, producing glucagon, insulin, somatostatin, or PP, differentiate from Pdx1(+) progenitors that transiently express Neurogenin3. To understand whether the competence of pancreatic progenitors changes over time, we generated transgenic mice expressing a tamoxifen-inducible Ngn3 fusion protein under the control of the pdx1 promoter and backcrossed the transgene into the ngn3(-/-) background, devoid of endogenous endocrine cells. Early activation of Ngn3-ER (TM) almost exclusively induced glucagon' cells, while depleting the pool of pancreas progenitors. As from E11.5, Pdx1(+) progenitors became competent to differentiate into insulin(+) and PP+ cells. Somatostatin(+) cells were generated from E14.5, while the competence to make glucagon(+) cells was dramatically decreased. Hence, pancreas progenitors, similar to retinal or cortical progenitors, go through competence states that each allow the generation of a subset of cell types. We further show that the progenitors acquire competence to generate late-born cells in a mechanism that is intrinsic to the epithelium.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available