Journal
DEVELOPMENTAL BIOLOGY
Volume 303, Issue 1, Pages 191-201Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2006.11.001
Keywords
DMP1 transgenic mice; odontogenesis; dentin apposition rate; tooth development; mineralization
Categories
Funding
- NIAMS NIH HHS [P01 AR046798-01A1, P01 AR046798, AR 046798, R01 AR051587-03, AR 51587, R01 AR051587] Funding Source: Medline
- NIDCR NIH HHS [K22 DE016977-02, T32 DE007294, T32 DE 07294, DE 016977, K22 DE016977, K22 DE016977-01A1] Funding Source: Medline
- PHS HHS [MT 11360] Funding Source: Medline
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Dentin matrix protein 1 (DMP1) is expressed in both pulp and odontoblast cells and deletion of the Dmp1 gene leads to defects in odontogenesis and mineralization. The goals of this study were to examine how DMP1 controls dentin mineralization and odontogenesis in vivo. Fluorochrome labeling of dentin in Dmp1-null mice showed a diffuse labeling pattern with a 3-fold reduction in dentin appositional rate compared to controls. Deletion of DMP1 was also associated with abnormalities in the dentinal tubule system and delayed formation of the third molar. Unlike the mineralization defect in Vitamin D receptor-null mice, the mineralization defect in Dmp1-null mice was not rescued by a high calcium and phosphate diet, suggesting a different effect of DMP1 on mineralization. Re-expression of Dmp1 in early and late odontoblasts under control of the Colla1 promoter rescued the defects in mineralization as well as the defects in the dentinal tubules and third molar development. In contrast, re-expression of Dmp1 in mature odontoblasts, using the Dspp promoter, produced only a partial rescue of the mineralization defects. These data suggest that DMP1 is a key regulator of odontoblast differentiation, formation of the dentin tubular system and mineralization and its expression is required in both early and late odontoblasts for normal odontogenesis to proceed. (c) 2006 Elsevier Inc. All rights reserved.
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