Journal
ACS CHEMICAL BIOLOGY
Volume 2, Issue 3, Pages 167-170Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cb600429k
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Funding
- NCI NIH HHS [R01 CA073808, R01 CA073808-09, CA73808] Funding Source: Medline
- NIGMS NIH HHS [R01 GM044783-09S1, R01 GM044783, GM44783] Funding Source: Medline
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We report on a means to endow proteins with the ability to permeate mammalian cells without appending an exogenous domain. Our approach is to install a cationic patch on the surface of a target protein by the grafting of arginine residues. Doing so with GFP did not compromise conformational stability but enabled efficient cellular uptake that was dependent on cell-surface glycosaminoglycans. We anticipate that this cell-permeable variant of GFP, which obviates the need for transfection, will be useful for numerous applications in cell biology and that the method of arginine grafting will be broadly applicable.
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