Journal
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
Volume 616, Issue 1-2, Pages 201-209Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mrfmmm.2006.11.025
Keywords
CHEK2; breast cancer; DNA damage; mouse model; genomic instability
Funding
- NCI NIH HHS [CA 90934] Funding Source: Medline
- NIEHS NIH HHS [P39-ES06096, U02 ES011038, ES07250] Funding Source: Medline
Ask authors/readers for more resources
Allelic variants of CHEK2 contribute to an elevated risk for human breast cancer and possibly other cancer types. In particular, the CHEK2*1100delC polymorphic variant has been identified as a low-penetrance breast cancer susceptibility allele in breast cancer families with wild type BRCA1 and BRCA2. To better understand the molecular basis by which this allele increases risk for disease, we have generated a mouse in which the wild type CHEK2 (Chk2 in mouse) allele has been replaced with the 1100delC variant. Mouse embryo fibroblasts (MEFs) derived from these mice have an altered cell cycle profile in which a far greater proportion of cells are in S-phase and in G2 (4N) compared with wild type cells. The mutant cells show signs of spontaneous genomic instability as indicated by polyploidy and an increase in DNA double strand breaks. (c) 2006 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available