Journal
JOURNAL OF PROTEOME RESEARCH
Volume 6, Issue 3, Pages 996-1005Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr060460c
Keywords
iTRAQ; LC-MS/MS; high light; Prochlorococcus; photoadaptation
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Funding
- Engineering and Physical Sciences Research Council [EP/E036252/1, GR/S84347/01] Funding Source: researchfish
- EPSRC [EP/E036252/1] Funding Source: UKRI
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In this study, we conducted biological and technical replicate proteomic experiments using isobaric tags for relative and absolute quantification (iTRAQ), to elucidate the light adaptation strategies of Prochlorococcus marinus MED4. The MED4 strain is adapted to an oceanic environment characterized by low nutrient levels, and ever-changing light intensities. Approximately 11% of the proteome was identified, with an average coefficient of variation of iTRAQ quantification values of 0.15. Fifteen proteins were deemed to be statistically and significantly differentially expressed in changing light intensities, particularly the down-regulation of photosystem-related proteins, and the up-regulation of the stress-related chaperone GroEL in high light compared to low light.
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