Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 45, Issue 3, Pages 1049-1052Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02426-06
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A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
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