Journal
MOLECULAR BIOTECHNOLOGY
Volume 35, Issue 3, Pages 215-223Publisher
HUMANA PRESS INC
DOI: 10.1007/BF02686007
Keywords
edible vaccine; Escheria coli heat labile toxin; fusion protein; endosperm-specific expression; porcine epidenic diarrhea virus
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Epitopes often co-delivery with adjuvant and targeting proteins to enable recognition by the immune system, and this approach may also increase the efficacy of the antigen. In this study, e assess and describe the ability of transgenic rice plant to express a fusion protein consisting of the B-subunit of the Escherichia coli heat-labile enterotoxin (LTB) and a synthetic core-neurilizing epitope (COE) of porcine epidemic diarrhea virus (PEDV), inducing an enteric disease that is seen most predominantly in piglets. Both components of the fusion proteins were detected with Western blot analysis. The fusion protein was determined to assemble into pentamers, as was evidenced by its ability to bind to GM(1) gangliosides, and evidenced an average level of expression in a transgenic rice endosperm. This indicates that the expression system of the plant is capable of generating a sizable amount of antigen, possibly allowing for the successful development of an edible vaccine.
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