Metallothionein and hsp70 expression in HepG2 cells after prolonged cadmium exposure

Cadmium is a widely distributed industrial and environmental pollutant. Principle target organs are soft tissues such as the liver, where cadmium accumulates with a biological half-life of approximately 20-30 years causing a variety of toxic responses. In HepG2, CdCl2 exposure for short periods (from I to 24 h) induces differential expression of stress proteins, including NIT and hsp70. However, less is known about the stress response during a prolonged exposure to this metal. MTT assay showed a low cytotoxicity of CdCl2 (0.1, 0.5, 1, 2, 5, 1 mu M), over a period of 72h. Cadmium uptake by ICP-AES technique and the corresponding expression of stress proteins (NIT, hsp70) during the same prolonged time were also analysed. Results show that Cd was continuously and increasingly accumulated, at the highest of the concentrations tested. Metallothionein expression was up-regulated with a saturation curve at 48 as well as 72 h after CdCl2 exposure. High levels of NIT probably confer an acquired tolerance to the stress and protection against cell injury as demonstrated by low cytotoxicity values. On the contrary, the unchanged pattern of hsp70 expression suggests that this protective mechanism, unlike other members of the family, is less involved during CdCl2 prolonged exposure. (c) 2006 Elsevier Ltd. All rights reserved.