Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 137, Issue -, Pages 105-114Publisher
SPRINGER
DOI: 10.1007/s12010-007-9043-5
Keywords
enzyme; ethanol; immobilized lipase; methanol; solvent; soybean oil
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The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyrne RM IM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best conditions were obtained in a solvent-free system with ethanol/oil molar ratio of 3.0, temperature of 50 degrees C, and enzyme concentration of 7.0% (w/w). Three-step batch ethanolysis was most effective for the production of biodiesel. Ethyl esters yield was about 60% after 4 h of reaction.
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