Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 387, Issue 5, Pages 1939-1944Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-1100-3
Keywords
MALDI-MS; binary matrix; matrix background suppression; small molecule detection
Ask authors/readers for more resources
Application of matrix-assisted laser-desorption ionization mass spectrometry (MALDI-MS) to small-molecule detection is often limited, because of high matrix background signals in the low-mass region. We report here an approach in which a mixture of two conventional MALDI matrices with different proton affinity was used to suppress the formation of matrix clusters and fragments. Specifically, when acidic alpha-cyano-4-hydroxycinnamic acid (CHCA) and basic 9-aminoacridine (9-AA) were used as the binary matrix, fewer background matrix peaks were observed in both positive and negative-mode detection of small molecules. In addition, the presence of CHCA substantially reduced the laser fluence needed for analyte desorption and ionization; thus better signal-to-background ratios were observed for negatively charged inositol phosphates in complex plant extracts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available