4.4 Article Proceedings Paper

Production of biosurfactant by Pseudomonas aeruginosa grown on cashew apple juice

Journal

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 137, Issue -, Pages 185-194

Publisher

SPRINGER
DOI: 10.1007/s12010-007-9050-6

Keywords

biosurfactant; cashew apple juice; Pseudomonas aeruginosa; raw materials; rhamnolipid; fermentation

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In this work, the ability of biosurfactant production by Pseudomonas aeruginosa in batch cultivation using cashew apple juice (CAJ) and mineral media was evaluated. R aeruginosa was cultivated in CAJ, which was supplemented with peptone (5.0 g/L) and nutritive broth. All fermentation assays were performed in Erlenmeyer flasks containing 300 mL, incubated at 30 degrees C and 1.50 rpm. Cell growth (biomass and cell density), pH, and superficial tension were monitored vs time. Surface tension was reduced by 10.58 and 41% when P. aeruginosa was cultivated in nutrient broth and CAJ supplemented with peptone, respectively. These results indicated that CAJ is an adequate medium for growth and biosurfactant production. Best results of biosurfactant production were obtained when CAJ was supplemented with peptone.

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