Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 387, Issue 5, Pages 1899-1906Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-1065-2
Keywords
sarcosine; creatine; creatinine; amperometric; biosensor
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An amperometric biosensor for the determination of creatine was developed. The carbon rod electrode surface was coated with sarcosine oxidase (SOX) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. The SOX from Arthrobacter sp. 1-1 N was purified and previously used for creation of a creatine biosensor. The natural SOX electron acceptor, oxygen, was replaced by an [Fe(CN)(6)](3-)/[Fe(CN)(6)](4-) redox mediating system, which allowed amperometric detection of an analytical signal at +400-mV potential. The response time of the biosensor was less than 1 min. The biosensor showed a linear dependence of the signal vs. creatine concentration at physiological creatine concentration levels. The optimal pH in 0.1 M tris(hydroxymethyl)aminomethane (Tris)-HCl buffer was found to be at pH 8.0. The half-life of the biosensor was 8 days in 0.1 M Tris-HCl buffer (pH 8.0) at 20 degrees C.
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