Journal
NEUROSCIENCE
Volume 145, Issue 1, Pages 209-224Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2006.11.060
Keywords
Alzheimer's disease; glial activation; glycogen synthase kinase-3beta; mimetic FGL peptide; neural cell adhesion molecule; NCAM
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By means of i.c.v. administration of preaggregated oligomeric beta-amyloid (A beta)(25-35) peptide it was possible in rats to generate neuropathological signs related to those of early stages of Alzheimer's disease (AD). A beta(25-35)-administration induced the deposition of endogenously produced amyloid protein. Furthermore, quantitative immunohistochemistry demonstrated time-related statistically significant increases in amyloid immunoreactivity, tau phosphorylation, microglial activation, and astrocytosis, and stereological investigations demonstrated statistically significant increased neuronal cell death and brain atrophy in response to A beta(25-35). Finally, the A beta(25-35)-administration led to a reduced short-term memory as determined by the social recognition test. A synthetic peptide termed FGL derived from the neural cell adhesion molecule (NCAM) was able to prevent or, if already manifest, strongly reduce all investigated signs of A beta(25-35)-induced neuropathology and cognitive impairment. The FGL peptide was recently demonstrated to be able to cross the blood-brain-barrier. Accordingly, we found that the beneficial effects of FGL were achieved not only by intracisternal, but also by intranasal and s.c. administration of the peptide. Furthermore, FGL-treatment was shown to inhibit the activity of GSK3 beta, a kinase implicated in signaling regulating cell survival, tau phosphorylation and the processing of the amyloid precursor protein (APP). Thus, the peptide induced a statistically significant increase in the fraction of GSK3 beta phosphorylated on the Ser9-position, a posttranslational modification known to inhibit the activity of the kinase. Hence, the mode of action of FGL with respect to the preventive and curative effects on A beta(25-35)-induced neuropathological manifestations and cognitive impairment involves the modulation of intracellular signal-transduction mediated through GSK3 beta. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.
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