Journal
SCIENCE
Volume 315, Issue 5816, Pages 1270-1274Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1138527
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Funding
- NIGMS NIH HHS [R01GM675287, R01 GM061010, R01 GM061010-07, R01GM61010] Funding Source: Medline
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Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.
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