Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1143, Issue 1-2, Pages 199-206Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.01.002
Keywords
quantification of polyhydroxyalkanoate; boron trifluoride; transesterification; poly(3-hydroxybutyrate); carbon storage compounds
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Standard chromatographic methods for the quantification of bacterial poly(3-hydroxyalkanoate) (PHA) proved to be inappropriate for the analysis of medium-chain-length PHA (mcl-PHA). Transesterification catalyzed by protic acids is not quantitative for mcl-PHA under common conditions due to slow reaction kinetics and formation of side-products in case of functionalized side-chains. To circumvent these limitations, an improved method for the quantification of mcl-PHA by GC-FID was developed. Boron trifluoride in methanol was successfully applied to quantitatively methanolyse different mcl-PHA (recovery > 94%). This novel method is well-suited for the analysis of purified mcl-PHA as well as for mcl-PHA in biomass. (c) 2007 Elsevier B.V. All rights reserved.
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