Journal
CHEMBIOCHEM
Volume 8, Issue 4, Pages 453-458Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.200600392
Keywords
analytical methods; bis(diphenylphosphinyl)-naphthofluorescein; fluorescent probes; superoxide radical anions
Ask authors/readers for more resources
4',9'-Bis(diphenylphosphinyl)naphthofluorescein (PNF-1) has been designed and synthesized as a highly selective, sensitive, cell-permeable, red fluorescent probe for detecting O-2(center dot-) in biological systems. The design strategy for the probe is based on the nucleophilic mechanism of O-2(center dot-) to mediate deprotection of the probe to naphthofluorescein, the emission spectrum of which is just in the spectral region of low background fluorescence interference in biological systems. Upon treatment with O-2(center dot-), the probe exhibits a strong fluorescence response and high selectivity for O-2(center dot-), rather than other reactive oxygen species or biological compounds. A linear calibration curve for PNF-1 showed a detection limit of 0.1 nm O-2(center dot-). This new type of fluorescent probe allows nanomolar changes in O-2(center dot-) concentrations in living cells to be detected by confocal microscopy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available