Journal
FEBS LETTERS
Volume 581, Issue 5, Pages 815-820Publisher
WILEY
DOI: 10.1016/j.febslet.2007.01.049
Keywords
jasmonate : amino acid synthetase; substrate specificity; firefly luciferase super family; adenosine 5 '-tetraphosphate synthesis; Ap(3)A; Ap(4)A
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Jasmonate:amino acid synthetase (JAR1) is involved in the function of jasmonic acid (JA) as a plant hormone. It catalyzes the synthesis of several JA-amido conjugates, the most important of which appears to be JA-Ile. Structurally, JAR1 is a member of the firefly luciferase superfamily that comprises enzymes that adenylate various organic acids. This study analyzed the substrate specificity of recombinant JAR1 and determined whether it catalyzes the synthesis of mono- and dinucleoside polyphosphates, which are side-reaction products of many enzymes forming acyl similar to adenylates. Among different oxylipins tested as mixed stereoisomers for substrate activity with JAR1, the highest rate of conversion to lie-conjugates was observed for (+/-)-JA and 9,10-dihydro-JA, while the rate of conjugation with 12-hydroxy-JA and OPC-4 (3-oxo-2-(2Z-pentenyl)cyclopentane-l-butyric acid) was only about 1-2% that for (+/-)-JA. Of the two stereoisomers of JA, (-)-JA and (+)-JA, rate of synthesis of the former was about 100-fold faster than for (+)-JA. Finally, we have demonstrated that (1) in the presence of ATP, Mg2+, (-)-JA and tripolyphosphate the ligase produces adenosine 5'-tetraphosphate (NA); (2) addition of isoleucine to that mixture halts the NA synthesis; (3) the enzyme produces neither diadenosine triphosphate (ANA) nor diadenosine tetraphosphate (ANA) and (4) Ap(4)A cannot substitute ATP as a source of adenylate in the complete reaction that yields JA-Ile. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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