Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 354, Issue 2, Pages 613-618Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2007.01.031
Keywords
immunotherapy; multiple myeloma; gamma delta T cells; ICAM-1; bisphosphonate
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We evaluated the mechanism of recognition of myeloma cells by gamma delta T cells. The expanded gamma delta T cells killed RPMI8226 and U266 myeloma cells in a gamma delta T-cell dose-dependent manner. Pretreatment of myeloma cells with zoledronic acid or mevastatin showed that gamma delta T cells kill myeloma cells by recognizing the mevalonate metabolites. The expression level of intercellular cell adhesion molecule-1 (ICAM-1) on myeloma cells correlates with the cytotoxicity by gamma delta T cells. Pretreatment of RPMI8226 and U266 with an anti-ICAM-1 monoclonal antibody inhibited their cytolysis. Moreover, AMC-1 myeloma cells transfected with of human ICAM-1 cDNA were susceptible to gamma delta T cells compared to parental AMO-1 cells. In conclusion, gamma delta T cells recognize the mevalonate metabolites and ICAM-1 on myeloma cells. (c) 2007 Elsevier Inc. All rights reserved.
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