Journal
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume 70, Issue 2, Pages 253-261Publisher
ELSEVIER
DOI: 10.1016/j.jbbm.2006.09.012
Keywords
plant material; sample preparation; chromatographic analysis
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This paper discusses preparation techniques of samples of plant material for chromatographic analysis. Individual steps of the procedures used in sample preparation, including sample collection from the environment or from tissue cultures, drying, comminution, homogenization, leaching, extraction, distillation and condensation, analyte enrichment, and obtaining the final extracts for chromatographic analysis are discussed. The techniques most often used for isolation of analytes from homogenized plant material, i.e., Soxhlet extraction, ultrasonic solvent extraction (sonication), accelerated solvent extraction, microwave-assisted extraction, supercritical-fluid extraction, steam distillation, as well as membrane processes are emphasized. Sorptive methods of sample enrichment and removal of interferences, i.e., solid-phase extraction, and solid-phase micro-extraction are also discussed. (c) 2006 Elsevier B.V. All rights reserved.
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