Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 55, Issue 1, Pages 26-30Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2006.10.042
Keywords
classical swine fever virus (CSFV); E2 gene; Chlanmydomonas reinhardtii; chloroplast transformatiom; anti-disease protein; biointerface; plant gene engineering
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The expression of classical swine fever virus (CSFV) structural protein E2 in different vectors, which has been shown to carry critical epitopes, has been established. Here, we reported a Chlamydomonas reinhardtii chloroplast expression vector, P64E2, containing classical swine fever virus structural protein E2 gene, which was constructed and transferred to C. reinhardtii by biolistic bombardment method. The transformants were identified by PCR, Southern blotting, Western blotting after selecting on resistant media. ELISA quantification assay showed that the expressed E2 protein accumulated up to 1.5-2% of the total soluble protein. The results of the study on the immunological activity indicated that the protein E2 expressed in C. reinhardtii chloroplasts could elicit animal bodies to produce antibodies against protein E2. (C) 2006 Elsevier B.V. All rights reserved.
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