Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 160, Issue 2, Pages 187-196Publisher
ELSEVIER
DOI: 10.1016/j.jneumeth.2006.09.007
Keywords
traumatic brain injury; controlled cortical impact; magnetic resonance imaging; mouse model; sensorimotor cortex; lesion evolution; injury characterization; behavioral tests
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Funding
- NIA NIH HHS [R21 AG026482] Funding Source: Medline
- NICHD NIH HHS [P30 HD02528, P30 HD002528] Funding Source: Medline
- NINDS NIH HHS [R01 NS039123] Funding Source: Medline
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The present study establishes a new mouse model for traumatic brain injury (TBI), using an electromechanically driven linear motor impactor device to deliver a lateral controlled cortical impact (CCI) injury to the sensorimotor cortex. Lesion cavity size was measured, and inter-animal consistency demonstrated, at 14 days post injury. Qualitative information regarding damage progression over time was obtained by scanning with high field magnetic resonance imaging (MRI) at five time points following injury. Functional impairment and recovery were measured with the Rotarod, gridwalk and cylinder tests, and lesion cavity volume was measured post mortem with thionin-stained tissue sections. The study establishes the reliability of a linear-motor based device for producing repeatable damage in a CCI model, demonstrates the power of longitudinal MRI in studying damage evolution, and confirms that a simple battery of functional tests record sensorimotor impairment and recovery. (c) 2006 Elsevier B.V. All rights reserved.
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