Journal
TALANTA
Volume 71, Issue 4, Pages 1455-1463Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2006.07.030
Keywords
phenolic acids; MCR-ALS; LC-DAD; coelution
Categories
Ask authors/readers for more resources
Use of Multivariate Curve Resolution Alternating Least Squares (MCR-ALS) is evaluated in the analysis of nine phenolic acids, both in standards mixture samples and in strawberry juice samples, by liquid chromatography with diode array detection (LC-DAD). Chromatographic coelution problems either because of unknown matrix interferences or because of the increase of organic modifier to reduce chromatographic analysis times are investigated. pH (4.25) and proportion of organic modifier in acetonitrile-water ratios (11:89, v/v) used as mobile phases have been optimized for separation of mixture of nine phenolic acids. Results obtained in the resolution and quantitation of phenolic acids in standards mixture samples and strawberry samples at two proportions of organic modifier (11:89 and 40:60 acetonitrile-water (v/v) ratios) show that the proposed MCR-ALS approach reduces analysis times and solvent expenses and improves determinations in case of strong coelution. Limits on the use of MCR-ALS are investigated in the analysis of phenolic acids in strawberry samples. (c) 2006 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available