Biocompatible superparamagnetic iron oxide nanoparticle dispersions stabilized with poly(ethylene glycol)oligo(aspartic acid) hybrids

Methoxypoly(ethylene glycol)-oligo(aspartic acid) (MPEG-Asp(n)-NH2, n = 2-5) hybrid block copolymers were synthesized and used as stabilizers to prepare superparamagnetic Fe3O4 nanoparticles with magnetite as the inner core and and poly(ethylene glycol) as the hydrophilic outer shell. The aqueous dispersions of the nanoparticles were stable at pH 2-11 and in 1M NaCl solution, when repeat number, n, was 3 or more. Transmission electron microscopy showed that the nanoparticles, stabilized with MPEG-Asp(3)-NH2, were about 14 nm in diameter. Magnetic measurements indicated that MPEG-Asp(3)-NH2-coated iron oxide nanoparticles showed superparamagnetic behavior. Cell adhesion assay and in vitro cell viability/cytotoxicity studies showed that MPEG-Asp3-NH2-coated iron oxide nanoparticles had less effect on cell adhesion/viability and morphology, and less cytotoxicity compared with uncoated, poly (acrylic acid)-coated, and MPEG-poly(acrylic acid)-coated iron oxide nanoparticles. (c) 2006 Wiley Periodicals, Inc.