Journal
BIOSENSORS & BIOELECTRONICS
Volume 22, Issue 8, Pages 1825-1829Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.08.042
Keywords
horseradish peroxidase; redox mediators; Safranine O; neutral red; Spacer arm
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The present study aims at investigating the use of redox dyes as non-diffusional electron mediators in hydrogen peroxide biosensors using horseradish peroxidase (HRP). We observe that the two redox dyes Safranine O and Neutral Red covalently bound to HRP, efficiently mediate electron transfer from the active site of the enzyme to the electrode surface. Dyes bound to the enzyme using a spacer arm diaminohexane further enhance the electron transfer. The enzyme electrodes show a linear response to the concentration of H2O2 up to 500 mu M concentration and with a detection limit of around 50 mu M. The dyes can be used as coupled mediators to develop a successful electro-optical biosensor. (c) 2006 Elsevier B.V. All rights reserved.
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