Journal
ANALYTICAL CHEMISTRY
Volume 79, Issue 6, Pages 2446-2450Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac061698n
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Funding
- NIGMS NIH HHS [GM 08194, S06 GM008194] Funding Source: Medline
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Enzyme-based reagentless biosensors were developed using the model system of glucose dehydrogenase (GDH) and its nicotinamide adenine dinucleotide cofactor (NAD(+)). The biosensors were prepared following an approach similar to the concept of molecular imprinting. To this end, the N-1-carboxymethyl-NAD(+) species were covalently attached to polyamino-saccharide chains of chitosan (CHIT) and allowed to interact with GDH in an aqueous solution. The bioaffinity interactions between the NAD(+) and GDH were secured by cross-linking the system with the glutaric dialdehyde (GDI)-modified CHIT. Electron conductive films of such CHIT-NAD(+)-GDH-GDI-CHIT macrocomplexes (MC) were prepared on glassy carbon (GC) electrodes by adding carbon nanotubes (CNT) and evaporating water. Electrochemical analysis of the GC/CNT-MC electrodes revealed that, in contrast to the oxidase-based electrodes, they acted as oxygen-independent reagentless biosensors. The application of Nafion to such biosensors predictably improved their selectivity and, unexpectedly, enhanced their sensitivity by an order of magnitude.
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