Journal
BIOSENSORS & BIOELECTRONICS
Volume 22, Issue 8, Pages 1807-1810Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.07.008
Keywords
hollow porous thiol-functionalized poly(DVB-co-AA) nanospheres; gold nanoparticles; horseradish peroxidase; biosensor; self-assembly
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A novel third-generation biosensor for hydrogen peroxide (H2O2) was developed by self-assembling gold nanoparticles to hollow porous thiol-functionalized poly(divinylbenzene-co-acrylic acid) (DVB-co-AA) nanospheres. At first, a cleaned gold electrode was immersed in hollow porous thiol-functionalized poly(DVB-co-AA) nanosphere latex to assemble the nanospheres, then gold nanoparticles were chemisorbed onto the thiol groups of the nanospheres. Finally, horseradish peroxidase (HRP) was immobilized on the surface of the gold nanoparticles. The immobilized horseradish peroxidase exhibited direct electrochemical behavior toward the reduction of hydrogen peroxide. The resulting biosensor showed a wide linear range of 1.0 mu M-8.0 mM and a detection limit of 0.5 mu M estimated at a signal-to-noise ratio of 3. Moreover, the studied biosensor exhibited high sensitivity, good reproducibility, and long-term stability. (c) 2006 Elsevier B.V. All rights reserved.
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