Journal
JOURNAL OF IMMUNOLOGY
Volume 178, Issue 6, Pages 3688-3694Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.178.6.3688
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Funding
- NIAID NIH HHS [R01 AI034238, R01 AI 034238, R01 AI 051356, R01 AI051356-04, R01 AI051356] Funding Source: Medline
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Differentiation of active from latent tuberculosis (TB) is a major. challenge in the control of TB. In this study, PBMC from latent TB-infected subjects, TB patients, and tuberculin skin test-negative donors stimulated with the Mycobacterium tuberculosis (Mtb)-specific Ag, early secretory antigenic target 6, and mRNA for 45 immune-related genes was measured by quantitative real-time PCR. Univariate analysis showed significant differences in the expression of 10 genes (IFN-gamma, FOXP3, IL-1 alpha, IL-1 beta, IL-2, IL-6, IL-8, IL-12 alpha, IL-12 beta, and IL-24) in PBMC from TB patients vs latent TB-infected subjects (p < 0.01). Multivariate logistic regression and classification and regression tree analyses revealed that expression of three genes, IL-8, FOXP3, and IL-12 beta, is predictive for TB vs latent Mtb infection. Thus, measurement of Ag-specific expression of these three genes may offer a specific and noninvasive means of differentiating between latent Mtb infection and TB.
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