Single base pair mismatch detection using cationic conjugated polymers through fluorescence resonance energy transfer

A simple method using water-soluble conjugated polymers and a DNA intercalator has been proposed for single base pair mismatch detection with enhanced detection efficiency. Fluorescence resonance energy transfer (FRET) was used as an indicator for unwinding of dsDNA due to base pair mismatch at elevated temperatures. The optical amplification effect of the Us helps to achieve enhanced detection efficiency.