Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 49, Issue 15, Pages 2680-2698Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.200905352
Keywords
inhibitors; metabolomics; natural products; protein profiling.; proteomics
Categories
Funding
- Deutschen Forschungsgemeinschaft (DFG)
- DIG-Sonderforschungsbereich [SFB 749]
- Fonds der Chemischen Industrie
- Center for Integrated Protein Science Munich (CiPS<SUP>M</SUP>)
- German National Academic Foundation
Ask authors/readers for more resources
Activity-based protein profiling (ABPP) has matured into a standard method for the fast, sensitive, and selective identification of enzyme activity and inhibitors in proteomes. By using natural product based probes, the targets of many uncharacterized molecules can be easily identified in complex proteomes, and their exact function and mechanism of action understood. Natural products and their derivatives can also serve as pharmaceutical lead structures that impede essential components in the cell and their effects can be studied in biological assays. Since the complex regulatory processes in a cell go beyond mere transcription, translation, and activation, it is imperative to also identify the products of the active proteome - the metabolites and binding partners of individual enzymes and proteins. Therefore, methods by which the chemically complex metabolome can be characterized are necessary A series of interesting approaches have become available in recent years that enable the global investigation of enzyme-metabolite pairs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available