Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1145, Issue 1-2, Pages 118-122Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.01.052
Keywords
astaxanthin; carotenoids; Dioszegia; Phaffia; Rhodotorula; Sporobolomyces; torulene; torularhodin; Xanthophyllomyces
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A simple method for the extraction of carotenoid pigments from frozen wet cells of red yeasts (Basidiomycota) and their analysis by reversed-phase HPLC using a C-18 column and a water/acetone solvent system is described. Typical red yeast carotenoids belonging to an oxidative series from the monocyclic gamma-carotene to 2-hydroxytorularhodin and from the bicyclic beta-carotene to astaxanthin were separated. Pigment identity was confirmed by LC-atmospheric pressure chemical ionisation (APCI) mass spectrometry using similar chromatographic conditions. (c) 2007 Elsevier B.V. All rights reserved.
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