Journal
CELL
Volume 128, Issue 6, Pages 1231-1245Publisher
CELL PRESS
DOI: 10.1016/j.cell.2006.12.048
Keywords
-
Categories
Funding
- Intramural NIH HHS Funding Source: Medline
- NCI NIH HHS [R33 CA105829, R21CA116365-01, R21 CA116365, F32 CA108313, R33CA105829, F32CA108313] Funding Source: Medline
- NCRR NIH HHS [P41 RR008605, P41 RR 08605] Funding Source: Medline
- NHGRI NIH HHS [R01 HG001696, U01HG003151, U01 HG003151, HG001696] Funding Source: Medline
Ask authors/readers for more resources
Insulator elements affect gene expression by preventing the spread of heterochromatin and restricting transcriptional enhancers from activation of unrelated promoters. In vertebrates, insulator's function requires association with the CCCTC-binding factor (CTCF), a protein that recognizes long and diverse nucleotide sequences. While insulators are critical in gene regulation, only a few have been reported. Here, we describe 13,804 CTCF-binding sites in potential insulators of the human genome, discovered experimentally in primary human fibroblasts. Most of these sequences are located far from the transcriptional start sites, with their distribution strongly correlated with genes. The majority of them fit to a consensus motif highly conserved and suitable for predicting possible insulators driven by CTCF in other vertebrate genomes. In addition, CTCF localization is largely invariant across different cell types. Our results provide a resource for investigating insulator function and possible other general and evolutionarily conserved activities of CTCF sites.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available