Scalable, low-cost, hierarchical assembly of programmable DNA nanostructures

We demonstrate a method for the assembly of fully programmable, large molecular weight DNA complexes. The method leverages sticky-end re-use in a hierarchical fashion to reduce the cost of fabrication by building larger complexes from smaller precursors. We have explored the use of controlled non-specific and specific binding between sticky-ends and demonstrate their use in hierarchical assembly. We conclude that it is feasible to scale this method beyond our demonstration of a fully programmable 8960 kD molecular weight 8 x 8 DNA grid for potential application to complex nanoscale system fabrication.