Journal
VETERINARY MICROBIOLOGY
Volume 121, Issue 1-2, Pages 29-38Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2006.11.008
Keywords
Rift Valley fever (RVF) virus; recombinant nucleocapsid (N) protein; indirect IgM and IgG ELISA; diagnostic accuracy; ruminants
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Serodiagnosis of Rift Valley fever (RVF) currently relies on the use of live or inactivated whole virus as antigens. The recombinant nucleocapsid (N) protein of RVF virus was tested for diagnostic applicability in an indirect enzyme-linked immunosorbent assay (I-ELISA), using sera from experimentally infected sheep (n = 128), vaccinated sheep (n = 240), and field-collected sera from sheep (n = 251), goats (n = 362) and cattle (n = 100). The N-protein based I-ELISA performed at least as good as VN and HI tests. In goat the diagnostic sensitivity (D-Sn) and specificity (D-Sp) of the I-ELISA was 100% when using the anti-species IgG conjugate. Using protein G as a detection system, the D-Sn and D-Sp in goats were 99.4% and 99.5%, in sheep field sera both 100%, in cattle 100% and 98.3%, respectively. The I-ELISA based on recombinant N-protein has the potential to complement the traditional assays for serodiagnosis of RVF. Advantages of the N-protem are its safety, stability and cost-effectiveness in use and production. (c) 2006 Elsevier B.V. All rights reserved.
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