Journal
JOURNAL OF WOOD SCIENCE
Volume 53, Issue 2, Pages 143-146Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s10086-006-0827-y
Keywords
amino acid; cytochemical staining; Norway spruce; protein; thermomechanical pulp
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Two methods of cytochemical staining using Coomassie dye and Cu+-bicinchoninic acid, respectively, showed that there are proteins in thermomechanical pulp (TMP) of Norway spruce. Protein isolated from TMP was analyzed for amino acid composition. There was about twice the amount of acidic amino acid material compared with basic amino acids, and the presence of glucosamine indicated that the isolated polypeptides also contained glycoproteins. The presence of proteins in ray cells and fiber tracheids in TMP adds to the chemical heterogeneity of the structurally complex high-yield pulp.
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