Journal
ANALYTICAL CHEMISTRY
Volume 79, Issue 7, Pages 2695-2702Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac061678l
Keywords
-
Categories
Ask authors/readers for more resources
We introduce an electrochemical method for the directed in situ assembly of various specific oligonucleotide capture probes onto different sensing elements of a multielectrode array in the aqueous environment of a flow cell. Surface plasmon microscopy is utilized for the on-line recording of the various functionalization steps. Hybridization reactions between targets from solution to the different surface-bound complementary probes are monitored by surface plasmon field-enhanced fluorescence microscopy using targets that are either labeled with organic dyes or with semiconducting quantum dots for color-multiplexing. This study provides a new approach for the fabrication of (small) DNA arrays and the recording and quantitative evaluation of parallel hybridization reactions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available