Journal
LEUKEMIA RESEARCH
Volume 31, Issue 4, Pages 445-454Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2006.07.016
Keywords
leukemias; MDR; P-glycoprotein; MRP1; DiOC(2); rhodamine-123; flow cytometry
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The expression and activity of P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRPI) were analyzed in 178 leukemia samples. Rhodamine-123 (Rho-123) and DiOC(2) were used as substrate to evaluate efflux pump activity. Chronic myeloid leukemia (CML) exhibited a higher percentage of positivity using Rho-123 than DiOC(2) (p=0.000) as compared to other types of leukemia. Moreover, Rho-123 was able to detected Pgp positive cells in a higher proportion of samples than DiOC(2) samples (p=0.004). Similarly, MRPI positive cells were best detected by Rho-123 as opposed to DiOC(2) (p=0.003). The co-functionality of Rho-123 and DiOC2 was observed in 26 out of 105 (24.8%) leukemic samples. Co-expression between Pgp and MRPI was detected in 30 out of 56 (53.6%) samples. As a whole, when the same samples were analyzed, Rho-123 was able to detect Pgp positive cells in a higher proportion of samples than DiOC(2) (p=0.000)Similarly, MRPI positive cells were best detected by Rho-123 as opposed to DiOC(2) (p=0.007). Our results support the idea that Rho-123 is the substrate of choice for leukemic cells. (c) 2006 Elsevier Ltd. All rights reserved.
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