Journal
JOURNAL OF INORGANIC BIOCHEMISTRY
Volume 101, Issue 4, Pages 700-708Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2006.12.016
Keywords
ruthenium(II)) complex; polypyridyl figand; DNA-binding; photocleavage
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A novel polypyridyl ligand 2-(4'-benzyloxyphenyl)imidazo[4,5-f][1,10]phenanthroline (BPIP) and its complex [Ru(bpy)(2)(BPIP)](2+) (1) (bpy = 2,2'-bipyridine) and (2) [Ru(phen)(2)(BPIP)](2+) (phen = 1, 10-phenanthroline) have been synthesized and characterized by elemental analysis, electrospray mass spectra and H-1 NMR. The DNA-binding properties of the two complexes were investigated by spectroscopic and viscosity measurements. The results suggest that both complexes bind to DNA via an intercalative mode. Both complexes can enantioselectively interact with calf thymus DNA (CT-DNA) in a way. The A enantiomer of complex I is slightly predominant for binding to CT-DNA to the Delta enantiomer. Under irradiation at 365 nm, both complexes have also been found to promote the photocleavage of plasmid pBR 322 DNA. Inhibitors studies suggest that singlet oxygen (102) and hydroxyl radical ((OH)-O-center dot) play a significant role in the cleavage mechanism for both complexes. Moreover, the DNA-binding and photocleavage properties of both complexes were compared with that of [Ru(bpy)(2)(BPIP)](2+) and [Ru(phen)(2)(BPIP)](2+). The experimental results indicate that methene group existence or not have a significant effect on the DNA-binding and cleavage mechanism of these complexes. (c) 2007 Elsevier Inc. All rights reserved.
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