Journal
ACS CHEMICAL BIOLOGY
Volume 2, Issue 4, Pages 247-251Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cb6003959
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Funding
- NCRR NIH HHS [1S10RR022393-01] Funding Source: Medline
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A convenient new method is described for the introduction of ketone groups at the N-termini of antibodies. The reaction occurs in the presence of pyridoxal-5-phosphate under conditions mild enough to maintain antigen binding function, as confirmed by enzyme-linked immunosorbent assay. Further derivatization of these functional sites was accomplished through oxime formation, yielding well-defined antibody conjugates for a wide range of applications. The ability of the modified antibodies to bind their targets was confirmed via immunodot blot analysis. The generality of this method has been demonstrated on a number of monoclonal and polyclonal antibodies, all with different binding specificities.
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