Journal
MICROBIOLOGY-SGM
Volume 153, Issue -, Pages 1286-1296Publisher
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/mic.0.2006/003483-0
Keywords
-
Categories
Ask authors/readers for more resources
Rhizobium tropici CIAT899 displays intrinsic tolerance to acidity, and efficiently nodulates Phaseolus vulgaris at low pH. By characterizing a gshB mutant strain, glutathione has been previously demonstrated to be essential for R. tropici tolerance to acid stress. The wild-type gshB gene region has been cloned and its transcription profile has been characterized by using quantitative real-time PCR and transcriptional gene fusions. Activation of the gshB gene under acid-stress conditions was demonstrated. gshB is also induced by UV irradiation. Upstream from gshB a putative sigma(70) promoter element and an inverted repeat sequence were identified, which are proposed to be involved in expression under neutral and acidic conditions, respectively. Gel retardation assays indicate that transcription in acid conditions may involve protein binding to an upstream regulatory region.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available