Journal
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 30, Issue 5, Pages 380-391Publisher
ELSEVIER
DOI: 10.1016/j.ejps.2006.12.006
Keywords
M cells; follicule associated epithelium; nanoparticles; oral delivery of peptides; Caco-2
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An alternative in vitro model of human follicle-associated epithelium (FAE) to study nanoparticle transport mechanisms by M cells was developed and characterized. The previous in vitro model of human FAE has been improved by inverting inserts after Caco-2 cell seeding. Raji and M cells were identified only in inverted co-culture cell monolayers by immunohistochemistry confocal microscopy, and electron microscopy. The M cell conversion rate evaluated by scanning electron microscopy ranged between 15 and 30% of cells. Transport of 200nm carboxylated polystyrene nanoparticles was higher and more reproducible in the inverted model. Nanoparticle transport was temperature-dependent, not affected by the presence of EGTA or by potassium depletion, but inhibited by EIPA or nystatin, suggesting that it occurs most likely by macropinocytosis. The inverted model appears more physiologic, functional and reproducible than the normally oriented model. (c) 2007 Elsevier B.V. All rights reserved.
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