Journal
BLOOD
Volume 109, Issue 7, Pages 2871-2877Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2006-07-036863
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Indoleamine 2,3-dioxygenase (IDO) is a novel immunosuppressive agent expressed in some subsets of normal and neoplastic cells, including acute myeloid leukemia (AML) cells. Here, we show that IDO expression correlates with increased circulating CD4(+)CD25(+)FOXP(3+) T cells in patients with AML at diagnosis. In vitro, IDO+ AML cells increase the number of CD4(+) CD25(+) T cells expressing surface CTLA-4 and FOXP3 mRNA, and this effect is completely abrogated by the IDO inhibitor, 1-methyl tryptophan (1-MT). Purified CD4(+)CD25(+) T cells obtained from coculture with IDO+ AML cells act as T regulatory (T-reg) cells because they do not proliferate, do not produce interleukin (IL)-2, and inhibit naive T-cell proliferation. Coculture with IDO(+)AML cells results in the conversion of CD4(+)CD25(-) into CD4(+)CD25(+) T cells, which is completely abrogated by 1-MT. Moreover, in mice, intrasplenic injection of IDO+ leukemia/ lymphoma A20 cells induces the expansion of bona fide T-reg cells by conversion of CD4(+)CD25(-) T cells; this effect is counteracted by 1-MT treatment. These data indicate that AML cells induce T-cell tolerance by directly converting CD4(+)CD25(-) T cells into CD4(+)CD25(+) T-reg cells through an IDO-dependent mechanism.
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