Journal
CHEMOSPHERE
Volume 67, Issue 8, Pages 1677-1680Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2006.11.051
Keywords
agricultural wastes; culture medium optimisation; inducers; static cultures; stainless steel sponges; submerged fermentation
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In the present study, we investigated the effect of different carbon sources (glucose, glycerol and ground mandarin peelings) on laccase production by Trametes pubescens grown on stainless steel sponges under static conditions. The cultures with ground mandarin peelings gave the highest laccase activities, showing values of about 100 U 1(-1). This is a very interesting result, since mandarin peelings are common agricultural wastes in some regions such as Mediterranean and Asiatic countries. Therefore, their reutilisation, besides reducing medium cost, also helps to solve the pollution problems caused by their disposal. Also, we studied the effect of supplementing the culture medium with different potential laccase-inducing compounds (ABTS, Tween 20, soya oil, Malaquite Green, Cu2+, tannic acid) on laccase production. Soya oil was the best inducer of laccase activities, attaining values 4-fold higher than those obtained in the reference cultures. (c) 2006 Elsevier Ltd. All rights reserved.
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